Enzymatic Determination of Cholesterol in Serum Lipoproteins
نویسندگان
چکیده
منابع مشابه
Enzymatic determination of total serum cholesterol.
An enzymatic method is described for determination of total serum cholesterol by use of a single aqueous reagent. The method requires no prior treatment of sample and the Calibration curve is linear to 600 mg/dl. Cholesterol esters are hydrolyzed to free cholesterol by cholesterol ester hydrolase (EC 3.1.1.13). The free cholesterol produced is oxidized by cholesterol oxidase to cholest-4-en-3-o...
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We describe a reliable, straightforward procedure for measuring the cholesterol content of serum lipoproteins separated by electrophoresis on cellulose acetate. The lipoprotein fractions are made visible by use of an enzymic reagent and then quantitated by densitometry. Standard electrophoresis equipment is used and the procedure requires just over 1 h for completion. All major fractions except...
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We investigated the immunoprecipitation of apolipoprotein B-binding lipoproteins, or of apo A-I- and apo C-binding lipoproteins, by delipidated antiserum for measuring cholesterol in the nonprecipitated lipoprotein fractions. After immunoprecipitation of serum with delipidated anti-apo B, we determined by immunoelectrophoresis that no beta- or pre-beta lipoproteins were present, whereas alpha-l...
متن کاملFluorometric enzymatic determination of total cholesterol in serum.
We describe a fluorometric enzymatic method for determining total serum cholesterol, based on hydrolysis of cholesterol esters to free cholesterol by cholesterol ester hydrolase (EC 3.1.1.13). The free cholesterol formed, as well as that initially present, is then oxidized by cholesterol oxidase (EC 1.1.3.6) to cholest-4-en-3-one with simultaneous production of hydrogen peroxide. The latter cat...
متن کاملLipoproteins, Cholesterol and Serum Proteins
over a period of 5 months for comparison with levels in people who develop mlyocardial infarction. We have studied 107 normal men for 7 years. Among these 11 developed infarction and 6 angina pectoris. Little change in lipid levels was associated with these events. Electrophoretic patterns early after infarction showed certain relatively characteristic changes, especially increase in a-2 and /3...
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ژورنال
عنوان ژورنال: Clinical Chemistry and Laboratory Medicine
سال: 1976
ISSN: 1434-6621,1437-4331
DOI: 10.1515/cclm.1976.14.1-12.217